The NEBNext RNA Library Prep Kit for MGI is designed to fulfil the requirement of producing dependable and superior directed RNA libraries for MGI sequencing applications.

The package includes reliable NEBNext master mixed reagents and methods, enabling the utilisation of a broad spectrum of inputs, ranging from 10 ng to 1 µg of total RNA. Prior to using the kit, one can employ rRNA depletion or poly(A) enrichment techniques. The NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) has an Express protocol, as described in the product description, which can save time.



Please be aware that the adaptors, primers, and circularisation reagents are included as independent components in the NEBNext Multiplex Oligos for MGI (Dual Index Primer Pairs Set 1) (NEB #E9725) and NEBNext Circularisation Module for MGI (NEB #E9720).