Every archaea and bacterium species possesses a 16S gene responsible for encoding the tiny subunit of ribosomes. This gene contains sections (V1 to V9) that exhibit significant variability and are utilised for taxonomical categorisation purposes. The gene also possesses conserved portions that can act as targets for primers to amplify the exceedingly variable regions.



The 16S rRNA approach is currently commonly employed to analyse intricate microbial communities, thanks to the implementation of Next-generation Sequencing (NGS). Next-generation sequencing (NGS) is the most effective method for studying any microbiome due to the limitations of Sanger sequencing and culture-based techniques. Sanger sequencing is unable to detect many bacteria in a single sample, while culture-based methods are time-consuming and often unable to cultivate certain bacterial species.



The EasySeq™ 16S Microbiome Library Prep Kit utilises Reverse Complement PCR (RC-PCR) technology to offer a cost-effective and efficient methodology for characterising the microbiome. This kit allows for the analysis of the hypervariable regions V3-V4 in a single reaction, all in one tube. The primary rationale for utilising the V3-V4 areas is their high nucleotide variability and superior discriminatory capability, which are essential for assessing bacterial diversity and quantifying microbial abundance in diverse sample types. The EasySeq™ 16S Microbiome Library Prep Kit has a high sensitivity, allowing for the detection of even very low levels of bacteria.